Work Package 3
>> Detection and Classification<<
To improve existing and potentially develop novel detection and classification techniques for anaerobic fungi in environmental as well as experimental samples, existing approaches (qPCR, visualization) shall be validated and improved, and novel approaches such as FISH, live/dead staining, PCR-based techniques, metagenomics/- transcriptomics and direct RNAseq shall be developed and evaluated. Alongside, an optimized bioinformatics workflow for taxonomic and functional analyses shall be established, that can then be used to describe and phylogenetically assign novel strains.
This work package will be led by the Bavarian State Research Center.
Hyphae and monocentric sporangia of the anaerobic fungus Neocallimastix frontalis adhering to and growing on hay (acid fuchsin staining). From Leis et al. 2014 .