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Eva Kofler

Bachelor Thesis 2024/25

Investigating Enzymatic Potential In Anaerobic
Gut Fungi: Protocol Establishment For
Characterization Of Xylanase Activity In
Selected Fungal Strains

This bachelor’s thesis investigated the enzymatic potential of

four anaerobic gut fungi for breaking down plant biomass,

with a focus the optimization of the DNS-assay for measuring

the xylanase activity. The adapted assay enabled robust,

comparative assessment of xylanase activity across strains

and showed clear differences in enzyme activity between the

strains. These findings highlight strain-specific differences

relevant for biotechnological applications.

Master Thesis 2025/26

Description of novel anaerobic fungal strains of
the phylum Neocallimastigomycota and
qualitative assessment of the accompanying
microbiota within their hosts

This Master’s thesis focuses on the characterization of novel

anaerobic gut fungal strain, using a combination of

microbiological and molecular methods. The strain’s growth

on different carbon sources is investigated both through gas

pressure measurement and microscopy. Furthermore, the

microbiome of its host-animal will be analyzed, contributing

to a better understanding of anaerobic gut fungal diversity

and their ecology.

We are a team of scientists from Austria, Germany and Switzerland working closely together combining microbiology, biotechnology and bioinformatics to investigate potential benefits deriving from the biotechnological usage of anaerobic fungi.

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© 2023

Impressum

Sabine Podmirseg: 

Tel: +43 (0) 512 507 51321

e-mail:sabine.podmirseg@uibk.ac.at

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